PRODUCTION AND APPLICATION OF MULTI-MONOCISTRONIC VECTOR USED IN SYNTHETIC BIOLOGY

摘要

The present invention relates to an expression cassette represented by the sequence number 1, a multi-monocistroric expression vector capable of expressing a plurality of different genes in one vector by the expression cassette, and a method for produce 2-deoxystreptamine using the multi-monocistroric expression vector. The expression cassette according to the present invention solves problems occurring in incomplete transcription and a problem of mRNA degradation and enables repetitive transcription, thereby improving gene expression. Also, the multi-monocistroric expression vector according to the present invention does not create problems of stop codon creation and frame shift occurring in case of recombining a plurality of genes with one vector, thereby being a very convenient tool for gene recombination and especially useful for biosynthesis of the 2-deoxystreptamine requiring recombination of multiple genes at one time.;COPYRIGHT KIPO 2014