METHOD FOR INHIBITION OF TERATOMA FORMATION BY SELECTIVELY INDUCED-APOPTOSIS OF HUMAN UNDIFFERENTIATED INDUCED PLURIPOTENT STEM CELLS

摘要

The present invention relates to a production method for induced-pluripotent-stem-cell-derived differentiated cells, from which undifferentiated induced pluripotent stem cells (iPS) have been eliminated, the method comprising the steps of: (a) preparing a cell sample comprising undifferentiated induced pluripotent stem cells and differentiated cells by differentiating induced pluripotent stem cells; and (b) selectively causing the death of the undifferentiated induced pluripotent stem cells by subjecting the product of step (a) to processing either with quercetin of formula 1 below or with YM-155 of formula 2 below. According to the present invention, the present invention makes it possible to effectively selectively cause the death only of undifferentiated induced pluripotent stem cells by causing induced pluripotent stem cells to differentiate into specific differentiated cells and then carrying out culturing in a differentiating culture medium comprising quercetin or YM-155, and, in the induced pluripotent stem cell differentiation method according to the present invention, only undifferentiated induced pluripotent stem cells that are a cause of teratoma formation are selectively caused to die, and thus differentiated differentiating cells are completely unaffected. In other words, the invention can be expected to ensure safety as the possibility of tumour formation during clinical use as a cell therapeutic agent is eliminated since the survival and functioning of the differentiated cells is maintained unchanged.