EFFICIENT MULTIPLEX PCR METHOD USING FLUORESCENT MARKERS AND MOBILITY MARKERS

摘要

The present invention relates to an efficient multiplex PCR method using fluorescent markers and mobility markers, and more specifically, to a multiplex PCR method, in which two primers are used to attempt at least two PCRs having different targets in one reaction liquid, and at least one primer included in each primer set is marked by a different marker of each primer set, thereby performing multiplex PCRs for differentiating PCR amplification products according to targets, wherein the combination of markers according to the primer set is a combination of 6-FAM, JOE, ALEXA 546, and ALEXA 568. According to the present invention, the PCR amplification products for respective markers can be clearly differentiated even in the multiplex PCRs for at least four markers, so that when it needs to be confirmed whether or not a plurality of target DNAs or RNAs are present, such as genetic testing, identification of organisms in the sample, or diagnoses of virus infection, accurate results can be obtained in a fast and convenient manner. Particularly, even in the case where a short repeat sequence is targeted, it can be confirmed whether or not effective amplification occurs.;COPYRIGHT KIPO 2014