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MOLECULAR MARKER FOR SELECTING CUCUMBER DOWNY MILDEW DISEASE RESISTANT VARIETY AND SELECTION METHOD USING THE SAME MARKER

机译:黄瓜霜霉病抗病品种选择的分子标记及使用该标记的选择方法

摘要

The present invention relates to a molecular marker for selecting cucumber downy mildew disease resistant entities and to a selecting method using the same. Provided is a polymorphic marker for selecting cucumber downy mildew disease resistant entities consisting of 10-100 continuous base sequences or the complementary base sequences of the same, wherein the 10-100 continuous base sequences includes one or more bases selected from a 36th base, a 238th base, a 349th based, or a 352th based in a base sequence represented by sequence number 1. Moreover, provided are a primer set which can amplify the polymorphic marker and selects cucumber downy mildew disease resistant entities and a primer set consisting of one or more base sequences selected from the group consisting of sequence number 4 to sequence number 11 or complementary base sequences of the same. In addition, provided are a method for selecting cucumber downy mildew disease resistant entities using the primer sets and a method for growing cucumber downy mildew disease resistant entities. Also, provided is a kit which includes the primer sets and restriction enzymes and selects cucumber downy mildew disease resistant entities.
机译:本发明涉及用于选择黄瓜霜霉病抗性实体的分子标记物以及使用该分子标记物的选择方法。提供了一种多态性标记,用于选择由10-100个连续碱基序列或其互补碱基序列组成的黄瓜霜霉病抗性实体,其中10-100个连续碱基序列包括一个或多个选自第36个碱基,在由序列号1表示的碱基序列中,第238个碱基,第349个碱基或第352个碱基。此外,提供了可以扩增多态性标记并选择黄瓜霜霉病抗性实体的引物组和由一个或多个组成的引物组。另外的碱基序列选自序列号4至序列号11或相同的互补碱基序列。另外,提供了使用引物组选择黄瓜霜霉病抗性实体的方法和生长黄瓜霜霉病抗性实体的方法。另外,提供了一种试剂盒,其包括引物组和限制酶,并选择黄瓜霜霉病抗性实体。

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