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Probe, Probe Set For Food Borne Pathogens And The Detecting Method Thereof

机译:食物源性病原体的探针,探针组及其检测方法

摘要

PURPOSE: A method for detecting bacteria causing food poisoning using a probe for detecting the bacteria is provided to accurately detect the bacteria with specificity and to quickly and effectively detect and identify a plurality of bacteria causing food poisoning. CONSTITUTION: A probe for detecting bacteria causing food poisoning comprises: LHO(left hybridization oligonucleotide) probe containing a forward primer binding region, and LHS(left hybridization sequence) region which is specifically hybridized to a first region of a target sequence; and RHO(right hybridization oligonucleotide) probe containing a reverse primer binding region and RHS(right hybridization sequence) region which is specifically hybridized to a second region continuously connected to the first region. A probe set for detecting the bacteria causing food poisoning contains 2-10 probes.
机译:目的:提供一种使用检测细菌的探针来检测引起食物中毒的细菌的方法,以准确地特异性地检测细菌,并快速有效地检测和识别导致食物中毒的多种细菌。构成:一种检测引起食物中毒的细菌的探针,包括:LHO(左杂交寡核苷酸)探针,其包含正向引物结合区和与靶序列的第一区域特异性杂交的LHS(左杂交序列)区。包含反向引物结合区和RHS(右杂交序列)区的RHO(右杂交寡核苷酸)探针,所述RHS(右杂交序列)区特异性杂交到与第一区域连续连接的第二区域。用于检测引起食物中毒的细菌的探针组包含2-10个探针。

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