Method of Detecting NFAT Using Capillary Electrophoretic Mobility Shift Assay by Binding of DNA

摘要

PURPOSE: A method for detecting NFAT which is a biomarker for myocardial hypertrophy, using a capillary electrophoretic mobility shift assay by DNA binding is provided to effectively determine myocardial hypertrophy and to identify myocardial hypertrophy mechanism. CONSTITUTION: A method for detecting NFAT which is a biomarker for myocardial hypertrophy comprises: a step of reacting a quantitated nuclear extract and a fluorescent residue-labeled DNA in a buffer solution; a step of detecting DNA-conjugated NFAT using laser-induced fluorescence and isolating by capillary electrophoresis; and a step of quantitating detected activated T cell nuclear factor(NFAT) and a product thereof. The fluorescent residue-labeled DNA contains a probe with 5'-CAGCTAGGAAACAATTGGAAGTG-3' sequence. The probe specifically binds to NFAT.