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Novel Brazzein Variants Introduced a Disulfide Bond for Enhanced Stability
Novel Brazzein Variants Introduced a Disulfide Bond for Enhanced Stability
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机译:新型Brazzein变体引入了二硫键以增强稳定性
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摘要
The present invention relates to: a novel brazzein multiple variant of increased sweetness; a nucleic acid molecule coated with the variant; a recombinant vector including the nucleic acid molecule; host cells transformed into the recombinant vector; a method for producing the variant; and a food composition for increasing sugar levels, which includes the variant as an active ingredient. The brazzein variant of the present invention is at least about two million times as sweet as sucrose (sugar) in the same amount, and the increase of its sweetness is 25 times as sweet as that of the wild brazzein. Therefore, the brazzein variant of the present invention has enhanced thermostability, and can bring out sweetness with a small amount thereof, as well as can be used to replace sugar or other sweeteners in food.
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