Production of Transgenic Aves Using Sequences for Germ Cell-Specific Gene Expression

摘要

PURPOSE: The production of transgenic aves using sequences for germ cell-specific gene expression is provided to analyze the differentiation or development of the priordial germ cells of aves into germ cells. CONSTITUTION: A method for producing transgenic aves includes the following: the priordial germ cells of aves are transformed based on a genetic vector containing a DAZL promoter as a germ cell-specific promoter, a NANOG promoter as a stem cell-specific promoter, and nucleotide sequence which encodes protein of revelation purpose; the transformed priordial germ cells of the aves are injected into accept media; and the transformed product of the aves is obtained. The protein is operatively bonded to the promoters.