Primer composition for loop-mediated isothermal amplification reaction for detecting Honeysuckle yellow vein virus, and use thereof

摘要

PURPOSE: A primer composition for loop-mediated isothermal amplification reaction for quickly detecting honeysuckle yellow vein virus is provided to early detect the virus, and to establish a quick and efficient system for diagnosing the virus. CONSTITUTION: A primer set for loop-mediated isothermal amplification reaction for detecting honeysuckle yellow vein virus contains sequence numbers 1-4. The virus is selected from the group consisting of Korea mutant strains(GQ477135), Japanese mutant strains(AB236325), New Zealand mutant strains(FJ817425), UK1 mutant strains(NC_005807), and UK2 mutant strains(AJ543429). A primer composition for loop-mediated isothermal amplification reaction for detecting honeysuckle yellow vein virus contains the primer set. The composition further contains DNA polymerase, dNTPs, and a reaction buffer. A method for detecting honeysuckle yellow vein virus comprises: a step of isolating genome DNAs; a step of performing loop-mediated isothermal amplification reaction at 60-70 deg. C for 30 minutes-2 hours; and a step of detecting the amplified product.