Composition for Detection of M. tuberculosis Complex and Mycobacteria Genus and Simultaneous Detection Method for M. tuberculosis Complex and Mycobacteria Genus with Multiplex Real Time PCR Using the Same

摘要

The present invention (i) Mycobacterium tuberculosis-specific genes of IS6110 primers to target and / or probe, (ii) mycobacterial genus-specific genes of ITS (Inter-Transcriptional Spacer) primers to target and / or probe, and optionally (iii) provides a M. tuberculosis and mycobacteria in nine minutes detecting composition containing the primers and / or probes to target the plant-derived gene as an internal control (internal control). ; When performing multiple real time PCR using the composition according to the invention Mycobacterium tuberculosis, as well as through the one time of the reaction it is possible to detect mycobacteria in the clinical diagnosis can be quickly and easily with high reliability.