Process for the preparation of biologically active substances from BLOOD FOR CSD activation of proliferation of undifferentiated cells HUMAN BONE MARROW

摘要

FIELD: biotechnologies.;SUBSTANCE: in order to obtain a biologically active substance after electric stimulation of chicken heads, blood is taken and incubated in the mode with voltage of 100-120 V, current force of 3-5 A during 3-5 seconds. Then, a serum is separated. A polypeptide fraction with molecular weight of 150-170 kilodalton is extracted from it. The obtained peptide fraction is lyophilised and sterilised by irradiation in a linear electron accelerator with a dose of 28-32 kGy. The invention can be used to obtain a remedy providing stimulation of proliferation of non-differentiated cells of human marrow, which can be used at treatment of oncohematological and autoimmune diseases.;EFFECT: preserving ability of non-differentiated cells of marrow to differentiation in a patient organism owing to excluding an inhibiting effect of an agent that stimulates proliferation; increasing a proliferation speed of non-differentiated cells of human marrow; reducing complications determined by immunogenic activity of the agent that stimulates proliferation, at use of an agent obtained by means of the proposed method; cheapening, simplifying the method owing to using available raw material.;2 tbl, 3 dwg