METHOD FOR PRODUCING CELL-NATURAL KILLERS AND DENDRIT CELLS FROM HUMAN EMBRYONAL HEMANGIO REGIONS OBTAINED FROM STEM CELLS

摘要

1. A method for producing natural killer (NK) cells, including: producing hemangioblasts; culturing hemangioblasts on methyl cellulose and a first cytokine mixture containing IL2, IL3, IL6, IL7, IL15, SCF and FL; collecting cultured cells; cultivating the collected cells in a liquid medium containing human serum and a second mixture of cytokines consisting of IL7, IL15, SCF and FL to obtain NK cells. 2. The method according to claim 1, characterized in that methyl cellulose is methyl cellulose H4236.3. The method according to claim 1, characterized in that methyl cellulose is methyl cellulose H4536.4. The method according to claim 1, characterized in that the concentration of IL2 is about 5-10 ng / ml, IL3 is about 1-10 ng / ml, IL6 is about 1-10 ng / ml, IL7 is about 5-20 ng / ml IL15 is about 5-10 ng / ml, SCF is about 10-50 ng / ml, and FL is about 10-50 ng / ml. 5. The method according to claim 1, characterized in that the cultivation of hemangioblasts is carried out approximately 6-8 days. The method according to claim 1, characterized in that the cultivation of the collected cells is carried out approximately 14-21 days. The method according to claim 1, characterized in that it further includes a weekly replacement medium for updating the second mixture of cytokines. The method according to claim 1, characterized in that the hemangioblasts differentiate from human embryonic stem cells (hESC). The method according to claim 1, characterized in that hemangioblasts differentiate from induced pluripotent (iPS) cells. The method according to claim 1, characterized in that the NK cells are immature NK cells and are CD56 + and CD16-.11. The method according to claim 1, characterized in that the NK cells are mature NK cells and are CD56- and CD16 +, or CD56lo and CD16 + .12. Natural killer cells (NK) obtained by any of the methods according to claims 1-11.13. Pharmaceutically acceptable composition