METHOD FOR LIBRARIES SERIAL BILATERAL in PCR with degenerate primers

摘要

The invention is a method for constructing deletion derivatives of libraries based on gene PCR with random seed, characterized in that the analyzed gene in the form of a linear DNA strand breaks introduced by treatment of pancreatic DNase I at a dilution series of discontinuities extend treating polymerase I of E. coli in the absence of nucleotide triphosphates, are attached to a matrix of a random primer having a 3 'end 6-, 11- or 17-membered random sequence and the 5'-end - the constant region (20 nucleotides) are designed nny adapter primer annealing nucleotides by treatment with T4 DNA ligase, after which the formulation is held operating time library by PCR with adapter primer symmetrical.