METHOD FOR DETERMINING PENETRATION riboflavin cornea with an infrared Fourier transform spectroscopy EXPERIMENT

摘要

A spectroscopic method for determining the penetration of riboflavin into the cornea cadaver eyes in the experiment, including the removal or treatment of corneal epithelium to enhance its permeability, instillation on the cornea of ​​a 0.1% solution of riboflavin for 30 min, followed by isolation of the corneal disk, spectroscopy and spectroscopy riboflavin, and comparison of the spectrum of the cornea treated with riboflavin and riboflavin spectrum, characterized in that before spectroscopy epithelium, if it was not previously removed, is removed and Spectros- apy carried out by infrared Fourier transform spectroscopy in attenuated total internal reflection mode, and the penetration into the corneal stroma riboflavin evidenced by the appearance of bands in its infrared spectrum, characteristic of riboflavin.