A method for detecting bacteria of the genus Azospirillum, having common antigenic determinant in PART lipopolysaccharide

摘要

1. A method of detecting bacteria of the genus Azospirillum, having common antigenic determinants of lipopolysaccharide composition comprising: obtaining specific antibodies against lipopolysaccharides; definition of the "working" of antibody concentration on lipopolysaccharides "model strain" serological relationship which must be installed; growing cultures of the test strains Azospirillum genus bacteria in liquid media at 30 ° C and preparation of bacterial suspensions based grown Azospirillum spp; adding antibodies in the "working" the concentration of bacterial suspensions, followed by preparation of drugs for light microscopy at room temperature; estimate mobility when bacteria of the genus Azospirillum preparations microscopy; - identification of bacteria having a common determinant consisting of polysaccharides, decreasing mobility bacteria of the genus Azospirillum in the presence of antibodies against lipopolysaccharides "model strain", characterized in that the "working" antibody concentration using twice the concentration of antibodies in the presence of which still all homologous cells model strain bacteria of the genus Azospirillum in the field of view of the microscope; "Working" the concentration of antibodies in the presence of all cells which still homologous strain of bacteria of the genus Azospirillum, remain unchanged regardless of the batch obtained antibodies; to determine the "working" antibody concentration assess percent motile cells homologous bacterial strain Azospirillum genus in a field of view of the microscope; as the studied bacteria of the genus Azospirillum strains used 18-hour culture, diluted with 50 mM phosphate buffered saline (PBS, pH 7) to zna