TEST immunoassay for the determination of allergenic components in milk and dairy products

摘要

System Test immunoassay for the determination of allergenic components of milk and dairy products containing polystyrene microplate with sorbed allergenic protein or mixture of proteins, the solution of specific antibodies to the allergen protein or antibody mixture to allergenic proteins Diluent solution for washing the microplate solution, the solution antispecies immunoperoxidase conjugate, the substrate of peroxidase and hydrogen peroxide, characterized in that to determine the total content of the test samples Allergenic components, including both native allergenic proteins and products of their partial destruction preserved antigenic determinants and capable of binding with specific antibodies, conducted competitive incubation specific antibody solution to the allergen protein or antibody mixture to allergenic proteins with two allergen-containing preparations - test sample and sorbed to polystyrene microplate allergenic proteins or mixtures of proteins, the said incubation is carried out in a competitive kinetiches th mode 30 minutes at room temperature 20-25 ° C, for a sufficient degree of registration of the reaction is provided by the high affinity of the specific antibodies, having a binding constant of at least 10M, and preventing the influence of matrix samples for analysis results provided by the significant 250-fold dilution samples, possible due to the high affinity antibodies.