IMPROVED METHOD OF MUTAGENESIS WITH APPLICATION OF POLYETHYLENE GLYCOL-MEDIATED INTRODUCTION OF MUTAGENIC NUCLEIC BASES INTO PLANT PROTOPLASTS

摘要

FIELD: chemistry.;SUBSTANCE: invention relates to field of biochemistry, in particular to methods of targeted change of duplex acceptor DNA-sequence and improvement of efficiency of targeted mutagenesis in plant protoplasts. Claimed methods include introduction into protoplasts of plant cells of single-stranded mutagenic oligonucleotide, which in its composition has, at least, one wrong nucleotide with respect to duplex acceptor DNA-sequence, which is to be changed with application of PEG-mediated transformation.;EFFECT: invention makes it possible to increase frequency of targeted mutagenesis.;10 cl, 3 tbl