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PURIFICATION AND APPLICATION OF FACTOR, CONTRIBUTING TO WOUND HEALING
PURIFICATION AND APPLICATION OF FACTOR, CONTRIBUTING TO WOUND HEALING
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机译:净化和应用因子,有助于伤口愈合
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摘要
FIELD: chemistry.;SUBSTANCE: group of inventions relates to the field of biotechnology. Claimed is a method of purification of a factor, contributing to wound healing, which represents a hepatocyte growth factor (HGF). All stages of purification are carried out in the presence of antithrombin III (AT-III). In accordance with the claimed method carried out are: defrosting of the frozen HGF-containing source and removal of sediment from the defrosted source. After that, the obtained solution, which contains a supernatant and AT-III, is brought in contact with a carrier for affinity chromatography on an immobilised heparin. Then, the solution is separated from the carrier for affinity chromatography. The carrier is brought in contact with a desorption buffer with ionic strength sufficient for HGF desorption. The desorption buffer, containing HGF, AT-III and histidine-rich glycoprotein (HRGP) is collected. Also claimed are wound-healing compositions, which contain HGF, AT-III and/or HRGP, purified by the claimed method.;EFFECT: inventions make it possible to increase step-by-step output of the hepatocyte growth factor, with the hepatocyte growth factor being concentrated in eluate in the presence of AT-III.;26 cl, 2 tbl, 6 ex
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机译:领域:化学。发明领域:一组发明涉及生物技术领域。要求保护的是一种有助于伤口愈合的因子的纯化方法,该因子代表肝细胞生长因子(HGF)。纯化的所有阶段均在抗凝血酶III(AT-III)的存在下进行。根据所要求保护的方法,执行以下操作:对冷冻的含HGF的源进行除霜,并从除霜的源中去除沉积物。之后,使包含上清液和AT-III的所得溶液与载体接触,以在固定的肝素上进行亲和层析。然后,将溶液与载体分离以进行亲和层析。使载体与离子强度足以使HGF解吸的解吸缓冲液接触。收集含有HGF,AT-III和富含组氨酸的糖蛋白(HRGP)的解吸缓冲液。还要求保护的是通过要求保护的方法纯化的,包含HGF,AT-III和/或HRGP的伤口愈合组合物。效果:本发明使得通过肝细胞逐步增加肝细胞生长因子的输出成为可能。生长因子在AT-III存在下浓缩在洗脱液中; 26 cl,2 tbl,6 ex
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