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VERO CELL LYSIS PROTEINS, METHOD FOR PRODUCTION THEREOF AND KIT FOR DETERMINING HOST CELL PROTEINS FOR VERO CELLS CONTAINING LYSIS PROTEINS
VERO CELL LYSIS PROTEINS, METHOD FOR PRODUCTION THEREOF AND KIT FOR DETERMINING HOST CELL PROTEINS FOR VERO CELLS CONTAINING LYSIS PROTEINS
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机译:病毒细胞裂解蛋白,其生产方法和试剂盒,用于测定含有裂解蛋白的病毒细胞的宿主细胞蛋白
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摘要
FIELD: chemistry.;SUBSTANCE: group of inventions relates to biotechnology and a method of producing proteins that are released during lysis of Vero cells, the method comprising the following steps: culturing and collecting Vero cells; break-down and lysis of Vero cells; purifying proteins released during lysis of Vero cells by hydrophobic chromatography on phenylsepharose 6FF, exclusive chromatography on sepharose, elution and concentration; the released proteins are a mixture of proteins with molecular weight of 11 kDa, 17-34 kDa, 55 kDa and 72-170 kDa. The proteins released during lysis of Vero cell according to the present invention have a wide range of applications. Said proteins can be used to control and analyse quality when producing vaccines using Vero cells with high sensitivity and good reproducibility. The present invention also provides a kit for analysing Vero cell HCP, characterised by high specificity, high sensitivity and good reproducibility.;EFFECT: kit can be used to control and analyse quality when producing not only non-secreted viral vaccines against viruses such as the hepatitis A virus, but other vaccines obtained using Vero cells as well.;18 cl, 14 tbl, 14 ex, 6 dwg
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