METHOD FOR ESTIMATING BIFIDUS BACTERIA AND LACTIC ACID BACILLI SURVIVAL IN EXPERIMENTAL ANIMAL'S GASTROINTESTINAL TRACT

摘要

FIELD: medicine.;SUBSTANCE: method for estimating the bifidus bacteria and lactic acid bacilli in experimental animal's gastrointestinal tract involves preparing bacterial mutants on dense nutrient media with increasing rifampicin concentrations from 10 mcg·ml^-1. The grown spontaneous mutants are selected, and the dense nutrient rifampicin medium is re-inoculated in the concentration of up to 160 mcg·ml^-1. The mutants characterised by hereditarily stable rifampicin resistance are selected for administration into laboratory animals in the concentration of 150-160 mcg·ml^-1 in the nutrient medium. The amount of the administered mutants is calculated. After keeping the animals, the selected faeces are suspended in isotonic sodium chloride. A supernatant is selected, and the dense nutrient medium containing rifampicin 110 mcg·ml^-1 is inoculated in a Petri dish. It is incubated for 72 h in the microaerophilic environment at 37°C. The plate count is measured, and the growth-positive bacteria count is re-calculated per 1 g of faeces. A portion of the growth-positive microorganisms of the administered count is determined and used to state the survival.;EFFECT: invention enables effectively determining the portion of the positive-growth bifidus bacteria and lactic acid bacilli passed through the animal's gastrointestinal tract.;2 dwg, 3 tbl, 3 ex