POLYSACCHARIDE-PROTEIN CONJUGATE POLYSACCHARIDE-PROTEIN FOR INDUCING IMMUNE RESPONSE AND PROTECTION AGAINST Staphylococcus aureus INFECTION, METHODS OF CONJUGATE OBTAINING (VERSIONS), CONJUGATE-CONTAINING COMPOSITION AND METHODS OF INDUCING IMMUNE RESPONSE AND PREVENTION OF Staphylococcus aureus INFECTION

摘要

FIELD: medicine, pharmaceutics.;SUBSTANCE: claimed group of inventions relates to field of immunology and deals with polysaccharide-protein conjugate, composition, containing such conjugate, method of inducing immune response against Staphylococcus aureus in subject, methods of obtaining such conjugate and method of reducing or prevention of Staphylococcus aureus infection. Characterised conjugate contains capsular Staphylococcus aureus. Said conjugate contains capsular polysaccharide, conjugated with protein carrier Said polysaccharide represents polysaccharide of serotype 5 or serotype 8, has molecular weight between 70 kDa and 800 kDa and degree of O-acetylation between 75 and 100%. Methods of obtaining charactersed comjugate include covalent conjugation of capsular polysaccharides with protein carrier by chemical method with application of either 1,1-carbonyl-di-1,2,4-triazole (CDT) or 3-(2-pyridylthio)propionylhydraside (PDPH).;EFFECT: claimed solutions make it possible to obtain vaccine preparations against infections caused by Staphylococcus aureus with higher immunogenicity.;29 cl, 11 dwg, 25 tbl, 20 ex