A method for the isolation of rna from volblutproben

摘要

A method for the isolation of rna from a whole blood sample comprising the steps of:a) contacting said sample with an aqueous analysis-solution containing at least one lysis substance in a concentration of 1.5 mol / l to 7 mol / l, at least one detergent and magnesium ions,b) simultaneous or subsequent addition of a proteinase,c) thereafter incubating the solution obtained after step b) for the at least partial enzymatic digestion and lysis of the sample, and wherein a lysated) to isolate the rna from the lysate by attachment to a solid phase.