method and device for simultaneous variable production and freely selectable optical superposition of phasenkontrast, polarization and fluorescence. light - bright - and dark field

摘要

method for production of a variable and simultaneous phasenkontrast and dark / hellfeld / polarisationsabbildung in microscope, characterized by the following steps of:the object with light, light from a device in the transmissive light and / or;- split up of light after the passage of the object or the reflection from the object to the image tiles into two separate, each assigned lichtstrahlenbu00fcndel methodenspezifischen information about the object.at least one of the lichtstrahlenbu00fcndel, change the tiles after phase, polarisationslage spectral composition and / or intensity;merge the image tiles overlap, and assigned to a summationsbild lichtstrahlenbu00fcndel through interference with the summationsbild in known manner by means of the okulars to betrachtbar or be documented.