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Brief description of the selection of clones of microbial strains or animal cells, in particular for the production of a protein
Brief description of the selection of clones of microbial strains or animal cells, in particular for the production of a protein
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机译:微生物菌株或动物细胞克隆的选择的简要说明,特别是用于蛋白质的生产
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摘要
The present invention relates to a process for the selection of clones of microbial strains or animal cells placed under conditions of inducing the production of proteins with a continuous supply of a solution containing the following compounds: at least a carbon-containing substrate, at least one nitrogen-containing compound, and one or more mineral salts, and the composition of the solution is chosen so that the elements of said compounds are in stoichiometric proportion with respect to the composition of the expected product, and that said compounds are in a form such that the ph is kept constant without regulation. The present invention applies particularly well to clones of strains used for the production of enzymes on the basis of a filamentous fungi, enzymes which are used for the production of ethanol from biomass ligno - cellulose.
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