VECTORS HAVING KOZAK SEQUENCES AND CELL INTRODUCED WITH THE SAME

摘要

PROBLEM TO BE SOLVED: To provide expression vectors of a gene for a heavy chain and a gene for a light chain, the vectors efficiently producing recombinants of an antibody by an appropriate combination of kozak sequences, a cell, and the like, that relate to a protein production technique using gene recombination.SOLUTION: The present invention comprises: introducing a vector containing a specific kozak sequence adjacent to a gene for a heavy chain of a rabbit-derived antibody and a vector containing a specific kozak sequence adjacent to a gene for a light chain of the rabbit-derived antibody, into a COS-1 cell; culturing the cell; and collecting produced antibodies.