METHOD FOR DESIGNING PRIMER FOR NUCLEIC ACID AMPLIFICATION, METHOD FOR PRODUCING THE SAME, PRIMER FOR NUCLEIC ACID AMPLIFICATION, PRIMER SET, AND METHOD FOR AMPLIFYING NUCLEIC ACID

摘要

PROBLEM TO BE SOLVED: To improve the speed and specificity of nucleic acid amplification in a method for isothermal nucleic acid amplification using turnback primer (TP).SOLUTION: As shown in Fig. (D1), in a target nucleic acid sequence, a sequence (A) is selected; and as an annealing sequence on the 3'-terminal side of a primer, a sequence (Ac') complementary to the sequence (A) is determined. As shown in Fig.(D2), in the target nucleic acid sequence, a sequence (B) present toward the 5'-terminal side of the sequence (A) is selected, and as a stem loop-forming sequence on the 5'-terminal side of the primer, the same sequence (B') as the sequence (B) is determined. As shown in Fig. (D3), in the target nucleic acid sequence, a sequence (W) between the sequence (A) and the sequence (B) and a sequence (Wc) complementary to the sequence (W) on the 3'-terminal side of the sequence (A) are selected; and as a sequence between the sequences (Ac') and (B') of the primer, the same sequence (W') as the sequence (W) is determined.