automatic microscope focus system and method for the analysis of transparent or low contrast of the sample

摘要

microscope systems and methods, to experimentally determine the boundary of the depth of field of the objective lens. The system and method largely automated, the operation of the sample to be imaged is executed by the processor and devices associated therewith. Calculation of experimental depth of field is also automated as well. Once the boundaries of the depth of field is determined experimentally, samples, especially when transparent or translucent, may be accurately imaged with a small user-defined depth than the depth of field is there.BACKGROUND 1