Prokaryotic light-induced gene expression system and method for regulating and controlling gene expression

摘要

The present invention provides a light-induced gene expression system for one prokaryotic bacterium, and a) a recombinant light-sensitive transcription factor coding gene comprising a first polypeptide as a DNA-binding domain and a second polypeptide as a light-sensitive domain; B) a promoter of at least one response element identified / bound by said first polypeptide, or a target-transcription unit comprising a promoter-response element, or a response element-promoter and a transcription-waiting nucleic acid sequence. The present invention further includes a prokaryotic expression vector including the corresponding light-induced gene expression system, a method for adjusting and controlling gene expression in the prokaryotic bacterium using the light-induced gene expression system, and each of the light-induced gene expression system A reagent kit is provided in which the components are charged. The prokaryotic bacterial photo-induced gene expression system of the present invention is rapid in induction, efficient and strong, safe with other inducing agents, less toxic or non-toxic, and can control the expression of genes in time and space, It is also used to adjust the life activity of prokaryotic bacteria.