Screening assays for the identification and validation of drug to the cancer stem cell targeting

摘要

Selectively cancer stem cells (CSCs) is the target, but the method of identification and validation of drug that does not have any effect on normal stem cells (NSCs) are described. Drugs, the drug inhibits cell growth of cancer stem cells surrogate (sCSC), or induces cell differentiation or death, not have the same effect on normal stem cells are identified by the ability. Cancer stem cell substitutes (sCSC) won the naturally transformed characteristics, but is a human embryonic stem cell (t-hPSC) or transformation induced pluripotent stem cell that holds the pluripotent (t-iPSC) . Dopamine receptor (DRs) are identified as a biomarker of cancer stem cells. Dopamine receptor blockers induce differentiation of cancer stem cells (CSCs), to eradicate the ability of the cancer stem cells to seed cancer (CSCs). .BACKGROUND 27