Dye set for surface-enhanced resonance Raman spectroscopy

摘要

The present invention relates to a kit for use in a multiplex assay, the kit comprises a dye set consisting essentially of a plurality of dyes, and the related product of the dye to the reference concentration. Dye sets, using surface enhanced resonance Raman spectroscopy, set the dye is in the presence of any other dyes of the set, these 0.6 reference density of each dye 1.5 2 One of over a range of concentrations of the dye in the dye, the sensitivity of 90% and is selected to be well distinguishable from the specificity 90%. In one embodiment, the dye sets of the following dyes, namely JOE, rhodamine green, ATTO520, BODIPY FL, BODIPY TMR-X, FAM, HEX, Cy3, Cy3.5, TAMRA, and consists TYE563. Furthermore, the present invention is a multiplex assay comprising a set of dyes, multiplex assay device for implementing, and a method of selecting a set of dyes.