How to characterize the interaction site on a target protein

摘要

The present invention relates to multiple improved and integrated methods for characterizing interaction sites on target proteins that modulate the phenotype of mammalian cells (such as non-lethal and / or non-proliferative phenotypes). . Such methods of the invention include methods for identifying target proteins that modulate such phenotypes in mammalian cells, and optionally for characterizing interaction sites on the target protein. Such identification and characterization methods are useful in the development of research tools and / or therapeutics, such as therapeutics using such proteins / peptides or small molecules. Accordingly, the present invention also relates to the following methods: identification of ligands (such as small molecule ligands) that bind to such target proteins; and identification of candidate compounds for modulators of the above phenotypes in mammalian cells. The present invention further provides peptides or proteins (or fragments, variants and / or derivatives thereof) comprising a specific amino acid sequence, nucleic acids encoding such peptides or proteins, and uses of such peptides or proteins or such The use of such nucleic acids. [Selection] Figure 10