Vector expressing simultaneously 12-mer TRAIL and HSV-TK suicide gene, and anti-cancer stem cell therapeutic agent using the same

摘要

The present invention includes a DNA cassette comprising a nucleic acid sequence encoding 12-mer TRAIL and a nucleic acid sequence of a suicide gene, a recombinant expression vector comprising the DNA cassette, a recombinant adenovirus produced using the recombinant expression vector, The present invention relates to a host cell transfected with the recombinant adenovirus, a cancer therapeutic composition containing the host cell, and a cancer therapeutic method comprising administering the cancer therapeutic composition to an individual. Since the stem cell therapeutic agent into which the DNA cassette of the present invention is introduced and produces 12-mer TRAIL and HSV-TK simultaneously has an excellent anticancer effect compared to conventional therapeutic agents, it can be used for the treatment of various solid cancers and metastatic cancers. It can be used effectively. [Selection] Figure 7