CELL CULTURE METHOD, PARTICULATE CULTURE CARRIER, AND PARTICLE-CONTAINING CELL AGGREGATE

摘要

PROBLEM TO BE SOLVED: To provide a culture method which can maintain cellular functions by improving permeability of nutrition, oxygen, a drug and a reagent from culture medium, while adhering a three-dimensional aggregate with the cellular function similar to organisms on a culture vessel.;SOLUTION: A cell aggregate containing particles can be formed while adhering cells to a culture vessel by giving micro scaffold particles having cell adhesion and substance permeability on the cell after plating isolated cells in the culture vessel and sedimenting the cells on a culture surface. For example, the cell aggregate containing particles is formed by giving gelatin particles with a size of 1/5 or more of a cell diameter, as a culture carrier contained in the cell aggregate, at the rate of a cell number: particle number is 80:1 or more based on the number of the cultured cells per unit culture area.;COPYRIGHT: (C)2015,JPO&INPIT