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method of detecting a target substance, aptamer set as well as sensors and devices used to it

机译:检测目标物质的方法,适配体以及用于该目标的传感器和设备

摘要

Method of detection of the target substance has the target substance in the sample for suffering inspection 1st aputama which (5) connects uniquely (1) and the target substance (5) 2nd aputama which 2nd ligand 6 other than connects uniquely (2), 1st aputama (1) has had two these chain formation regions which two these chains of 1 or more form, 2nd aputama (2) has had two these chain formation regions of 2 or more, the target substance as for base arrangement of 1st aputama 1 which (5) connects, two these chains of 1st aputama 1Portion of base arrangement of the formation region is included at least, base arrangement of 2nd aputama 2 which the 2nd ligand (6) connects 2nd aputama (2) includes the portion of base arrangement of two these chain formation regions of 2 or more at least, the mixture process and the target substance which (4) are prepared the process which (5) and 1st aputama (1) is made to connect and the process which makes with the 2nd ligand (6) and 2nd aputama 2 and, the process which detects the cleavage of two these chain formation regions of 1st aputama 1 and, is included.
机译:靶标物质的检测方法是在检查对象样品中的靶标物质具有唯一连接的(1)的第一痰(1)和具有唯一连接的第二配体6(2)以外的第二配体6的靶标物质(5),第一第1个aputama 1的碱基配置中,第2个aputama(2)具有两个以上的1个以上的链形成的两个这些链形成区域,第2个aputama(2)具有两个以上的2个以上的这些链形成区域。 (5)连接,这些第一个puta 1的两个链至少包括形成区域的基本结构的一部分,第二配体2连接的第2 aputama 2的基本结构(6)连接第二个puta 2(2)的基本结构的一部分这些至少2个以上的链形成区域中的两个,(4)的混合步骤与目标物质(5)与第1aputama(1)相连的步骤,与第二配体形成的步骤(6)和第2口腔2,该方法包括检测第一个putama 1的两个这些链形成区的断裂的过程。

著录项

  • 公开/公告号JP5803923B2

    专利类型

  • 公开/公告日2015-11-04

    原文格式PDF

  • 申请/专利权人 日本電気株式会社;

    申请/专利号JP20120531658

  • 发明设计人 田光 公康;

    申请日2011-06-17

  • 分类号C12Q1/68;G01N33/53;C12N15/115;C12M1/34;G01N33/543;G01N37/00;

  • 国家 JP

  • 入库时间 2022-08-21 15:30:22

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