Detection method and its use of the epitope of the allergen or the candidate

摘要

The disclosure herein provides a method of detecting a set of epitopes of two or more allergens in one. According to the disclosure herein, Upon detecting the candidate epitope or allergen, and the test sample group as (a) test sample containing antibodies from allergic positive individuals of multiple allergies negative individuals multiple the array per each of the control sample group containing an antibody that is derived from, overlapping peptides plurality of predetermined length based on the amino acid sequence or a portion thereof allergenic protein potential is fixed, the samples of each group supplies respectively, by contacting the antibody of said one or more with overlapping peptides of the plurality, each overlapping peptides of said plurality, detecting specific binding of the antibodies of said one or more and, there is overlapping peptide selected from the overlapping peptides of the plurality, is obtained from the test sample groups and the average control strength information about the intensity of the specific binding of the overlapping peptides derived from the control sample group or a first epitope of the allergen, the overlapping peptides of one or more on the basis of the average test intensity information about the intensity of the specific binding of the overlapping peptides, the specific binding is positive and the candidate relates test sample is selected from the test sample group, and the average control strength information and the test intensity information of the individual of the overlapping peptides corresponding to the candidate or a first epitope of said one or more When it can be based on the door, and the candidate epitope or first the overlapping peptides from the overlapping peptide other candidate other than the epitope or said first, and based on the average control strength information I want to be with the candidate or second epitope in the allergen the overlapping peptides of one or two or more with the individual test strength information that exceeds the critical value or greater than a value obtained Te.