Method and experimental equipment for quantitative optical measurements

摘要

The present invention is a method of quantitative optical measurement of the characteristic properties of at least one analyte in at least one laboratory sample, in particular a method of fluorescence measurement of at least one biochemical or biological sample. Wherein the method uses a laboratory instrument with at least one light source and at least one detector device, wherein the laboratory instrument has the ability of the laboratory instrument to detect a signal with at least one detector device. Utilizing at least one sensitivity parameter S to control, the method uses source light for causing at least one sample to emit sample light, and at least one detector device for detecting sample light And the method uses at least one sensitivity parameter S to detect the intensity I of the corresponding at least one sample light. The method comprises the following steps:-measuring at least one reference point (S_ref; I_ref);-measuring at least one first intensity I_m1 of the sample light assigned to the first analyte Using at least one first sensitivity parameter S_m1 that is not identical to S_ref;-determined by utilizing at least one reference point (S_ref; I_ref) and at least one measurement point (S_m1; I_m1) A quantity Q1 that is a measure of the slope of the line is determined; is dependent on -Q1 and is characteristic of the characteristics of the first analyte, in particular characterized by the concentration of the first analyte in the at least one sample The quantity Q1 is calculated in order to calculate the first analyte value C_m1 in particular according to the formula Q1 = (I_m1-I_ref) / (S_m1-S_ref) To use, including, it relates to the aforementioned method. The method further relates to a laboratory instrument configured to apply the method according to the invention.