Inspection method of gene copy number variation

摘要

The present invention genetic testing field , and in particular gene copy number variation method of inspection . In this method , select the array and ersatz in the source of or outside sources of similar sequences of inspection waiting gene , after you design the corresponding primer , was amplify sequences similar array of inspection waiting gene at the same time , the melting curve I analyze . When the copy number variation occurs in the inspection wait gene , analysis of its melting curve is that it can be clearly distinguished from the analysis of the wild -type gene inspection waiting , the purpose can be achieved in the test . The method of the present invention is not only examination of gene copy number variation , is also used for inspection of chromosomal aneuploidy . The method of the present invention the gene copy number variation patients and chromosomal aneuploidy screening patients , in addition to being applied before normal birth testing , also applies to non-invasive prenatal diagnosis . The method of the present invention is fast , accurate , high-sensitivity , high specificity , high throughput , low cost , has advantages such as no pollution . [ Selection Figure Figure 1