Method for synthesizing single-stranded cDNA, and detection methods of nucleic acid preparation of the microarray sample.

摘要

In order to provide a method of amplifying nucleic acid which can produce a nearly constant amount of amplified products irrespective of the amount of RNA to be used as a template, the primer is used at a final concentration of 500 pM to 500 nM in a reaction which synthesizes a single-stranded cDNA using RNA extracted from a biological sample as a template and using a primer with oligo dT and a promoter sequence.