A novel ultra sensitive cell based sensor and its use

摘要

Regarding sensors useful new cell-based, is the sensor, as a regulated secretory granules stored reporter polypeptide in cell lines with regulated exocytosis professional, the present invention, secretory granules were transfected by proteases in drug discovery include cell lines with regulated exocytosis professional, have any of the heterologous molecule or endogenous molecules as modulators of exocytosis of regulated secretory granules, the sensor, the granules storage such proteases at least, the reporter, high resistance to state that already exist, such as protein degradation and pH decrease due to other proteases, the granules; unstimulated or basic; cleavage sequence highly specific; enzyme activity of exocytosis after secretion of very low levels under these conditions; and cell culture viability, and for detection with high sensitivity and robustness of high-throughput, signal-to-background high in the medium that is compatible with the granule exocytosis I have activity. None [Selection Figure]