a method for manufacturing a α- galactose epitope expression virus and vaccine

摘要

A method for producing an ±-Gal-expressing virus having enhanced immune response to viruses, without requiring the use of any enzyme; an influenza virus vaccine having a high effect (antigenicity), which is produced using an ±-Gal-expressing virus produced by the method; and others. Specifically disclosed are: a method for producing an ±-galactose epitope (Gal±1-3Gal²1-4GlcNAc -R: ±-Gal hereinafter)-expressing virus, which comprises the steps of: (1) introducing an ±1,3-galactosyltransferase gene in an expressible condition into a cell line that does not express ±-Gal to obtain a cell line capable of expressing ±-Gal; (2) inoculating a virus into the cell line capable of expressing ±-Gal to obtain a cell line infected with a virus; and (3) culturing the cell line infected with the virus to obtain a virus expressing ±-Gal from the culture medium.