Porcine circovirus type 2 protective epitope densely carried by chimeric papaya ringspot virus–like particles expressed in <i xmlns='http://www.wiley.com/namespaces/wiley'>Escherichia coliEscherichia coli as a cost‐effective vaccine manufacture alternative

Aguilera Brenda Eugenia; Chávez‐Calvillo Gabriela; Elizondo‐Quiroga Darwin; Jimenez‐García Mónica Noemí; Carrillo‐Tripp Mauricio; Silva‐Rosales Laura; Hernández‐Gutiérrez Rodolfo; Gutiérrez‐Ortega Abel

摘要

Abstract > Porcine circovirus type 2 (PCV2) still represents a major problem to the swine industry worldwide, causing high mortality rates in infected animals. Virus‐like particles (VLPs) have gained attention for vaccine development, serving both as scaffolds for epitope expression and immune response enhancers. The commercial subunit vaccines against PCV2 consist of VLPs formed by the self‐assembly of PCV2 capsid protein (CP) expressed in the baculovirus vector system. In this work, a PCV2 protective epitope was inserted into three different regions of papaya ringspot virus (PRSV) CP, namely, the N‐ and C‐termini and a predicted antigenic region located near the N‐terminus. Wild‐type and chimeric CPs were modeled in silico , expressed in Escherichia coli , purified, and visualized by transmission electron microscopy. This is the first report that shows the formation of chimeric VLPs using PRSV as epitope‐presentation scaffold. Moreover, it was found that PCV2 epitope localization strongly influences VLP length. Also, the estimated yields of the chimeric VLPs at a small‐scale level ranged between 65 and 80 mg/L of culture medium. Finally, the three chimeric VLPs induced high levels of immunoglobulin G against the PCV2 epitope in immunized BALB/c mice, suggesting that these chimeric VLPs can be used for swine immunoprophylaxis against PCV2. </abstract> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> <div class="translation abstracttxt"> <span class="zhankaihshouqi fivelineshidden" id="abstract"> <span>机译：</span><Abstract XMLNS =“http://www.wiley.com/namespaces/wiley”type =“main”> <title type =“main”>抽象</ title> > porcine circovirus类型2（pcv2）仍然代表全世界猪业的一个主要问题，导致感染动物的死亡率高。病毒样颗粒（VLP）患有疫苗发育的注意力，作为表位表达和免疫应答增强剂的支架。对PCV2的商业亚基疫苗包括由在杆状病毒载体系统中表达的PCV2衣壳蛋白（CP）的自组装形成的VLP。在这项工作中，将PCV2保护表位插入番木瓜中的三种不同地区，即N-和C-Termini和位于N-末端附近的预测抗原区域。野生型和嵌合CPS在硅中进行建模，在大肠杆菌中表达，纯化，并通过透射电子显微镜观察和可视化。这是第一个显示使用PRSV作为表位呈现支架形成嵌合VLP的报告。此外，发现PCV2表位定位强烈影响VLP长度。此外，嵌合VLP的估计产量在小规模水平范围内范围为65至80mg / L的培养基。最后，三个嵌合VLP诱导了抵抗免疫BALB / C小鼠的PCV2表位的高水平免疫球蛋白G，表明这些嵌合VLP可用于对PCV2的猪免疫营养基。 </ p> </ abstract> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> </div> <div class="record"> <h2 class="all_title" id="enpatent33" >著录项</h2> <ul> <li> <span class="lefttit">来源</span> <div style="width: 86%;vertical-align: text-top;display: inline-block;"> <a href='/journal-foreign-15016/'>《Biotechnology and Applied Biochemistry》</a> <b style="margin: 0 2px;">|</b><span>2017年第3期</span><b style="margin: 0 2px;">|</b><span>共9页</span> </div> </li> <li> <div class="author"> <span class="lefttit">作者</span> <p id="fAuthorthree" class="threelineshidden zhankaihshouqi"> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Aguilera Brenda Eugenia&option=202" target="_blank" rel="nofollow">Aguilera Brenda Eugenia;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Chávez‐Calvillo Gabriela&option=202" target="_blank" rel="nofollow">Chávez‐Calvillo Gabriela;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Elizondo‐Quiroga Darwin&option=202" target="_blank" rel="nofollow">Elizondo‐Quiroga Darwin;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Jimenez‐García Mónica Noemí&option=202" target="_blank" rel="nofollow">Jimenez‐García Mónica Noemí;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Carrillo‐Tripp Mauricio&option=202" target="_blank" rel="nofollow">Carrillo‐Tripp Mauricio;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Silva‐Rosales Laura&option=202" target="_blank" rel="nofollow">Silva‐Rosales Laura;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Hernández‐Gutiérrez Rodolfo&option=202" target="_blank" rel="nofollow">Hernández‐Gutiérrez Rodolfo;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Gutiérrez‐Ortega Abel&option=202" target="_blank" rel="nofollow">Gutiérrez‐Ortega Abel;</a> </p> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zkzz" style="display: none;">展开▼</span> </div> </li> <li> <div style="display: flex;"> <span class="lefttit">作者单位</span> <div style="position: relative;margin-left: 3px;max-width: 639px;"> <div class="threelineshidden zhankaihshouqi" id="fOrgthree"> <p>Unidad de Biotecnología Médica y FarmacéuticaCentro de Investigación y Asistencia en Tecnología y Dise?o del Estado de Jalisco A.C.Normalistas 800 Colinas de la Normal Guadalajara Jalisco 44270 México;</p> <p>Departamento de Ingeniería GenéticaCentro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacionalkm 9.6 Libramiento Norte Carretera Irapuato‐León Irapuato Guanajuato 36821 México;</p> <p>Unidad de Biotecnología Médica y FarmacéuticaCentro de Investigación y Asistencia en Tecnología y Dise?o del Estado de Jalisco A.C.Normalistas 800 Colinas de la Normal Guadalajara Jalisco 44270 México;</p> <p>Laboratorio de la Diversidad BiomolecularCentro de Investigación y de Estudios Avanzados del Instituto Politécnico NacionalLibramiento Norte km 9.6 Carretera Irapuato‐León Irapuato Guanajuato 36821 México;</p> <p>Laboratorio de la Diversidad BiomolecularCentro de Investigación y de Estudios Avanzados del Instituto Politécnico NacionalLibramiento Norte km 9.6 Carretera Irapuato‐León Irapuato Guanajuato 36821 México;</p> <p>Departamento de Ingeniería GenéticaCentro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacionalkm 9.6 Libramiento Norte Carretera Irapuato‐León Irapuato Guanajuato 36821 México;</p> <p>Unidad de Biotecnología Médica y FarmacéuticaCentro de Investigación y Asistencia en Tecnología y Dise?o del Estado de Jalisco A.C.Normalistas 800 Colinas de la Normal Guadalajara Jalisco 44270 México;</p> <p>Unidad de Biotecnología Médica y FarmacéuticaCentro de Investigación y Asistencia en Tecnología y Dise?o del Estado de Jalisco A.C.Normalistas 800 Colinas de la Normal Guadalajara Jalisco 44270 México;</p> </div> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zhdw" style="display: none;">展开▼</span> </div> </div> </li> <li > <span class="lefttit">收录信息</span> <span style="width: 86%;vertical-align: text-top;display: inline-block;"></span> </li> <li> <span class="lefttit">原文格式</span> <span>PDF</span> </li> <li> <span class="lefttit">正文语种</span> <span>eng</span> </li> <li> <span class="lefttit">中图分类</span> <span><a href="https://www.zhangqiaokeyan.com/clc/180.html" title="生物工程学（生物技术）">生物工程学（生物技术）;</a><a href="https://www.zhangqiaokeyan.com/clc/177.html" title="生物化学">生物化学;</a></span> </li> <li class="antistop"> <span class="lefttit">关键词</span> <p style="width: 86%;vertical-align: text-top;"> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=capsid protein&option=203" rel="nofollow">capsid protein;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=epitope carrier&option=203" rel="nofollow">epitope carrier;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=IgG response&option=203" rel="nofollow">IgG response;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=papaya ringspot virus&option=203" rel="nofollow">papaya ringspot virus;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=porcine circovirus 2 epitope&option=203" rel="nofollow">porcine circovirus 2 epitope;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=virus‐like particles&option=203" rel="nofollow">virus‐like particles;</a> </p> <div class="translation"> 机译：衣壳蛋白;表位载体;IgG反应;番木瓜ringspot病毒;猪圆环病毒2表位;病毒样粒子; </div> </li> </ul> </div> </div> <div class="literature cardcommon"> <div class="similarity "> <h3 class="all_title" id="enpatent66">相似文献</h3> <div class="similaritytab clearfix"> <ul> <li class="active" >外文文献</li> <li >专利</li> </ul> </div> <div class="similarity_details"> <ul > <li> <div> <b>1. </b><a class="enjiyixqcontent" href="/journal-foreign-detail/0704021881128.html">Porcine circovirus type 2 protective epitope 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Porcine circovirus type 2 protective epitope densely carried by chimeric papaya ringspot virus–like particles expressed in Escherichia coliEscherichia coli as a cost‐effective vaccine manufacture alternative

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