Mold detection method, PCR reaction solution, and mold detection carrier

摘要

In a test for confirming the presence or absence of mold, it is possible to specifically detect a wide variety of molds with high accuracy. A mold detection method comprising a step of amplifying a DNA fragment containing a target region in mold DNA and confirming the presence or absence of an amplification product, wherein the target region is an ITS region and a β-tubulin gene To do. In the PCR reaction solution for performing amplification of the target region, the concentration ratio of the primer set for amplifying the β-tubulin gene and the primer set for amplifying the ITS region is 1: 0.9-1 : 0.1.