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MUTANT NEQ HS DNA POLYMERASE DERIVED FROM NANOARCHAEUM EQUITANS AND ITS APPLICATION TO HOT-START PCR
MUTANT NEQ HS DNA POLYMERASE DERIVED FROM NANOARCHAEUM EQUITANS AND ITS APPLICATION TO HOT-START PCR
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机译:源自纳米古菌的突变NEQ HS DNA聚合酶及其在热启动PCR中的应用
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摘要
A DNA polymerase (Neq DNA polymerase) derived from Nanoarchaeum equitans is split into Neq L and Neq S fragments, each of which contains inteins. A Neq hot-start (HS) DNA polymerase in which the inteins of the Neq L and Neq S fragments are linked with each other is provided in the form of a precursor of Neq DNA polymerase. A purification method can be significantly improved by inserting a His-tag sequence composed of six histidine residues between the inteins of the Neq L and Neq S fragments at a gene level. As a result of effort to enhance PCR efficiency of the Neq HS DNA polymerase, a gene coding for the Neq HS DNA polymerase is mutated at specific positions to screen mutant Neq HS polymerases (M1, M2, and M3) having a highly improved PCR amplification rate and amplification level.
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