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ULTRA-RAPID AND SENSITIVE DNA DETECTION USING DNAZYME AND ON-CHIP ISOTACHOPHORESIS
ULTRA-RAPID AND SENSITIVE DNA DETECTION USING DNAZYME AND ON-CHIP ISOTACHOPHORESIS
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机译:脱氧核糖核酸酶和芯片上同位素电泳技术进行超灵敏的DNA检测
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摘要
A DNA detection method combines DNAzyme reactions and on-chip isotachophoresis (ITP). A mixture of sample containing a target DNA and a DNAzyme sensor which is either (1) a catalytic molecular beacon or (2) a binary DNAzyme and a probe is loaded into a trailing electrolyte (TE) reservoir of a microfluidic chip. In the presence of the target DNA, the catalytic molecular beacon or the probe is cleaved to generate a fluorescent fragment. Enhanced DNAzyme reaction occurs at the TE-to-LE interface. Fluorescent signal from cleaved catalytic molecular beacon or probe is detected either at the location where DNAzyme reaction occurs or at a separate location. In the latter case, the microfluidic chip has a separation region containing a capture gel or a sieving matrix which allows the fluorescent fragment to pass through but captures or traps the uncleaved catalytic molecular beacon or probe.
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