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HOMOLOGOUS RECOMBINATION-BASED NUCLEIC ACID MOLECULAR CLONING METHOD AND RELATED KIT
HOMOLOGOUS RECOMBINATION-BASED NUCLEIC ACID MOLECULAR CLONING METHOD AND RELATED KIT
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机译:基于同源菌重组的核酸分子克隆方法及相关试剂盒
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摘要
This invention provides a homologous recombination-based nucleic acid molecular cloning method. According to the method of the present invention, a target DNA is cloned into a vector through homologous recombination by providing a linearized vector with both ends respectively added with a sequence (namely, a target DNA-specific homologous arm) homologous with sequences of both ends of the target DNA or a flank sequence thereof, or by utilizing a ligation fragment containing both the target DNA-specific homologous arm and a vector-specific homologous arm (a sequence homologous with a specific region of the vector). The method of the present invention is especially applicable to the cloning of a large DNA fragment and to the studies of single nucleotide polymorphism. The present invention further provides a related kit.
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