Methods for Simultaneous Quantification of Thyroid Hormones and Metabolites Thereof by Mass Spectrometry

摘要

The invention provides methods for simultaneously detecting or simultaneously quantifying any combination of thyroxine (T₄), triiodothyronine (T₃), 3,3′-diiodo-L-thyronine (3,3′-T₂), 3-iodothyronamine (T₁AM), and, optionally, reverse T₃ (rT₃) in a sample obtained from a human. The method involves a simple, sensitive, accurate, and specific isotope dilution tandem mass spectrometry method for the simultaneous quantification of any combination of T₄, T₃, 3,3′-T₂, T₁AM, and, optionally, rT₃ in a sample obtained from a human, e.g., in human plasma or serum samples. This assay is far more sensitive than previously described assays for thyronamines and allows quantitation of T₁AM in human plasma or serum, including from healthy controls.