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Application of Oligo-DT Molecules To Avoid Generation of High Molecular PCR Products Induced By Poly-A Carrier
Application of Oligo-DT Molecules To Avoid Generation of High Molecular PCR Products Induced By Poly-A Carrier
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机译:Oligo-DT分子在避免聚A载体诱导的高分子PCR产物产生中的应用
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摘要
During RNA isolation carrier nucleic acids such as polyA-RNA are used in order to increase the yield of the isolated RNA. The carrier nucleic acids may lead to high molecular weight products which may interfere in subsequent steps, such as reverse transcription, PCR and gel electrophoresis. The present invention therefore refers to a method and a kit for reverse transcription and the use of a blocking nucleic acid molecule for blocking the carrier polyA-RNA.
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