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TEST METHOD FOR EFFICACY OF SILVER SURFACES AS A SANITIZER

机译:银表面消毒剂功效的测试方法

摘要

A method that includes the steps: inoculating nutrient agar with bacterial stock to form a culture; incubating the culture to form a first incubated culture; incubating a portion of the first culture with nutrient agar to form a second culture; incubating a portion of the second culture to form a third culture; incubating the third culture to form an inoculated test plate; forming an inoculum by suspending bacteria from the inoculated test plate in a buffered test solution, adjusting the pH to ˜7 to 8, and adding organic soil at a concentration of approximately 10% to 30% by weight; inoculating a silver-containing surface region of a test carrier with a portion of the inoculum; incubating the inoculated test carrier; washing the test carrier in a neutralizing solution to form a residual test inoculum; and calculating the percent reduction in the number of surviving bacterial colonies in the residual test inoculum.
机译:一种方法,包括以下步骤:用细菌原种接种营养琼脂以形成培养物;温育培养物以形成第一温育培养物;将第一培养物的一部分与营养琼脂一起孵育以形成第二培养物;孵育一部分第二种培养物以形成第三种培养物;孵育第三种培养物以形成接种试板;通过将来自接种的测试板的细菌悬浮在缓冲的测试溶液中,将pH调节至约7至8,并以约10重量%至30重量%的浓度添加有机土壤来形成接种物;用一部分接种物接种测试载体的含银表面区域;孵育接种的测试载体;在中和溶液中洗涤测试载体以形成残留的测试接种物;并计算残留测试接种物中存活细菌菌落数量减少的百分比。

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