ARTIFICIAL FORISOME BODY WITH SEO-F FUSION PROTEINS, PLANT OR YEAST CELLS COMPRISING VECTORS WHICH CODE FOR THESE PROTEINS, AS WELL AS VECTORS WHICH CODE FOR SEO-F FUSION PROTEINS

摘要

The invention relates to artificial forisome bodies comprising a fusion protein of at least one SEO-F protein or an at least 50-amino acid portion thereof, and at least one additional protein or peptide, with the exception of GFP and the Venus protein, wherein(a) the additional protein or peptide has a mass of at most 30 kDa, or(b) the forisome body further comprises an unfused SEO-F protein or a form of said protein having C-terminal deletions of up to 45 amino acids and/or N-terminal deletions of up to 13 amino acids, wherein the unfused SEO-F protein is selected from proteins having the property of being capable of forming homomeric forisome bodies in the absence of additional SEO-F proteins, or(c) the further protein or peptide is a portion of a second SEO-F protein, with the proviso that one of the two SEO-F proteins in its unfused form is capable of forming homomeric forisome bodies, and the fusion protein is comprised of an N-terminal SEO-F protein portion and a C-terminal SEO-F protein portion, wherein the portions represent a SEO-F protein that is complete or C-terminally deleted by up to 50 amino acids and/or N-terminally deleted up to 13 amino acids,with the proviso that optionally any number of the four cysteines located in the C-terminal portion between the aa 600 and aa 670 of the native SEO-F proteins may be replaced by amino acids that are not capable of forming a SS-bond.;The invention further relates to a plant cell or yeast cell, comprising a vector encoding said fusion protein and optionally additionally for the unfused SEO-F protein or the deleted form thereof as defined in (b), and a vector amplifiable in yeast, comprising a portion encoding said fusion protein.;The artificial forisome body of the invention is useful for protein-chemistry purposes, particularly for providing immobilized enzymes, antibodies, antigens, as well as such proteins or peptides that can be immobilized on a substrate due to their affinity reaction with a substrate-bound biological or biochemically produced material.