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Hybridization- Independent Labeling Of Repetitive DNA Sequence In Human Chromsomes
Hybridization- Independent Labeling Of Repetitive DNA Sequence In Human Chromsomes
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机译:人类染色体中重复DNA序列的杂交独立标记
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摘要
The present invention discloses a transcription activator-like effector-based strategy, termed “TALEColor”, for labeling specific repetitive DNA sequences in human chromosomes. TALEs were custom designed for human telomeric repeats and fused with any of numerous fluorescent proteins (FPs). TALE-telomere-FP fusion proteins were used to detect telomeric sequence in both living cells and fixed cells. Using human cells with different average telomere lengths, TALEColor signals correlated positively with telomere length. TALEs were also designed to detect centromeric sequences unique to specific chromosomes, enabling localization of these specific human chromosomes in live cells. These methods may have significant potential both for basic chromosome and genome research as well as in clinical applications.
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